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RuiJun Tian

Associate Professor

Department of Chemistry


09/2010 – 04/2014    CIHR postdoctoral fellow, proteomics and signal transduction Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital and University of Toronto, Toronto, ON, Canada

                 Advisor: Dr. Tony Pawson Co-advisor: Dr. Tony hunter (Salk) and Dr. Art Weiss (UCSF)       
07/2008 – 08/2010    Postdoctoral fellow, proteomics Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada 

                Advisor: Dr. Daniel Figeys       

09/2003 – 06/2008    PhD of Science, analytical chemistry CAS Key Lab of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences,Dalian, China 

                 Advisor: Dr. Hanfa Zou       
09/2002 – 06/2003    Graduate courses, analytical chemistry and biochemistry University of Science and Technology of China, Hefei, China       
09/1998 – 07/2002    Bachelor of Science, chemistry School of Chemistry and Chemical Engineering, Inner Mongolia University, Huhhot, China       
01/2001 – 07/2001    Exchange study, analytical chemistry and biochemistry Peking University, Beijing, China
09/2014    Peacock Program Awardee from Shenzhen Government
04/2012    2012 Young Investigator Award, International Association for Protein Structure Analysis and Proteomics (IAPSAP) 

2011-2013   Canadian Institutes of Health Research (CIHR) Fellowship   
09/2009    Young investigator travel award, HUPO 8th World Congress, Toronto       
08/2008    Excellent graduate, Graduate School of Chinese Academy of Sciences       
08/2008    President’s Scholarship, Chinese Academy of Sciences       
08/2007    Poster Award, the CNHUPO 5th Annual Congress, Guangzhou       
06/2007    “Best Student Leader”, Graduate School of Chinese Academy of Sciences       
06/2007    “Best student”, Graduate School of Chinese Academy of Sciences       
1998-2002    Scholarships of Inner Mongolia University, Hohhot (3 times)       
12/2000    Outstanding Individual of "Go to the Countryside" in Summer Holiday, Education department and Youth League committee of Inner Mongolia Government, Hohhot

·    Editorial Board:
Scientific Reports, Frontiers in Endocrinology, Austin Proteomics
·    Ad hoc Reviewer: 
Analytical Chemistry, TrAC Analytical Chemistry, Proteomics, Electrophoresis, Journal of Chromatography A, Analytical Methods, Cells
·    Membership: 
Canadian National Proteomics Network (CNPN), American Society for Mass Spectrometry (ASMS)


Apr. 16, 2015    Division of Life Science, Hong Kong University of Science and Technology, Hong Kong

Nov. 20, 2014    13th Young Instrumental Analysis Meeting, Xi’an
Aug. 25, 2014    5th Asia Oceania Mass Spectrometry Conference, Beijing
Jul. 17, 2014    College of Chemistry, Jilin University
May 22, 2014    Changchun Institute of Applied Chemistry, CAS
May 22, 2014    3rd China-Canada Symposium on Systems Biology and Personalized
May 18, 2014    Medicine
Nov. 2, 2013    College of Medicine, University of Saskatchewan, Canada
Oct. 12, 2013   Research Center for Eco-Environmental Sciences, CAS
Sep. 24, 2013   Department of Chemistry, Nankai University
Sep. 26, 2013   School of Life Sciences, USTC 
Apr. 30, 2013   Enabling Technologies (ETP 2013), Toronto
Mar. 28, 2013   Biochemistry and Microbiology Department, University of Victoria
Jun. 26, 2012    The 19th Methods in Protein Structure Analysis Meeting, Ottawa
May 8, 2012     “Probing Signalling Networks” Annual Meeting, Toronto
Aug. 29, 2011    3rd China-Canada Analytical Chemistry Conference, Toronto 
May 9, 2010    Canadian National Proteomics Network 2nd Symposium, Montreal       
Dec. 2, 2009    Ontario Proteomic Methods Centre General Meeting, Toronto       
Oct. 19, 2009    24th Interactional Symposium on Microscale Bioseparations, Dalian       
Sep. 26, 2009    HUPO 8th World Congress, Toronto       
Jun. 4, 2007    1st Dalian International Symposium and Exhibition on Chromatography, Dalian       
Dec. 28, 2006    International Symposium on Chemical Biology and Combinatorial Chemistry, Jinan       
Apr. 21, 2005    15th National Symposium and Exhibition on Chromatography, Zhengzhou  


PUBLICATIONS (h-index = 19; ResearcherID: J-3019-2014)

Corresponding-author and first-author papers:
1.    Ruijun Tian*‡, Haopeng Wang*, Gerald D. Gish, Evangelia Petsalaki, Adrian Pasculescu, Yu Shi, Marianne Mollenauer, Richard D. Bagshaw, Nir Yosef, Tony Hunter, Anne-Claude Gingras, Arthur Weiss‡, Tony Pawson, A combinatorial proteomic analysis of intercellular signaling applied to the CD28 T cell co-stimulatory receptor, Proc. Natl. Acad. Sci. U. S. A., 2015, 112, E1594–E1603
2.    Ruijun Tian‡, Exploring intercellular signaling by proteomic approaches, Proteomics, 14(4-5):498-512 
(Direct invitation by Editor-in-chief: Michael Dunn)
3.    Ruijun Tian*‡, Jing Jin*, Lorne Taylor, Brett Larsen, Susan E. Quaggin, Tony Pawson‡, Rapid and sensitive MRM-based mass spectrometry approach for systematically exploring ganglioside-protein interactions, Proteomics, 2013, 13, 1334–1338 (Direct invitation to RT)
- Highlighted as a key scientific article in Global Medical Discovery
4.    Ruijun Tian, Matias Alvarez-Saavedra, Hai-Ying M. Cheng, Daniel Figeys, Uncovering the proteome response of the master circadian clock to light using an AutoProteome system, Mol. Cell. Proteomics, 2011, 11, M110.00725 
5.    Ruijun Tian*, Shuai Wang*, Fred Elisma, Li Li, Hu Zhou, Lisheng Wang, Daniel Figeys, Rare cell proteomic reactor applied to stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics study of human embryonic stem cell differentiation, Mol. Cell. Proteomics, 2011, 10, M110.000679 (Cover paper) 
6.    Ruijun Tian, He Zhang, Mingliang Ye, Xiaogang Jiang, Lianghai Hu, Xin Li, Xinhe Bao, Hanfa Zou, Selective extraction of peptides from human plasma by highly ordered mesoporous silica particles for peptidome analysis, Angew. Chem. Int. Ed., 2007, 46, 962-965 
7.    Ruijun Tian*, Xuyen Dai Hoa*, Jean-Philippe Lambert, John Paul Pezacki, Teodor Veres, Daniel Figeys, Development of a multiplexed microfluidic proteomic reactor and its application for studying protein-protein interactions, Anal. Chem., 2011, 83, 4095-4102 
8.    Ruijun Tian*, Lianbing Ren*, Huaijun Ma, Xin Li, Lianghai Hu, Mingliang Ye, Ren’an Wu, Zhijian Tian, Zhen Liu, Hanfa Zou, Selective enrichment of endogenous peptides by chemically modified porous nanoparticles for peptidome analysis, J. Chromatogr. A, 2009, 1216, 1270-1278 
9.    Shuai Wang*, Ruijun Tian*, Li Li, Lisheng Wang, Daniel Figeys, An enhanced chemically defined SILAC culture system for quantitative proteomics study of human embryonic stem cells, Proteomics, 2011, 11, 4040-4046 
10.    Ruijun Tian*, Lindong Li*, Wei Tang, Hongwei Liu, Mingliang Ye, Zongbao K. Zhao, Hanfa Zou, Chemical proteomic study of isoprenoid chain interactome with a synthetic photoaffinity probe, Proteomics, 2008, 8, 3094–3104
11.    Ruijun Tian, Mingliang Ye, Xiaogang Jiang, Lianghai Hu, Xin Li, Hanfa Zou, Selective extraction of peptides in acidic human plasma by porous silica nanoparticles for peptidome analysis with 2-D LC-MS/MS, J. Sep. Sci., 2007, 30, 2204-2209
12.    Ruijun Tian, Xinning Jiang, Xin Li, Xiaogang Jiang, Shun Feng, Songyun Xu,Guanghui Han, Mingliang Ye , Hanfa Zou, Biological fingerprinting analysis of the interactome of a kinase inhibitor in human plasma by a chemiproteomic approach, J. Chromatogr. A, 2006, 1134, 134–142
13.    Ruijun Tian, Junming Sun, He Zhang, Mingliang Ye, Chuanhui Xie, Jin Dong, Jiwei Hu, Ding Ma, Xinhe Bao, Hanfa Zou, Large-pore mesoporous SBA-15 silica particles with submicrometer size as stationary phases for high-speed CEC separation, Electrophoresis, 2006, 27, 742–748 
14.    Ruijun Tian, Songyun Xu, Xiaoyuan Lei, Wenhai Jin, Mingliang Ye, Hanfa Zou, Characterization of small-molecule-biomicromolecule interactions: From simple to complex, Trac-Trend Anal.Chem., 2005, 24, 810-825


Co-author papers:

1.    Harrod H. Ling, Christian Beaulé, Cheng-Kang Chiang, Ruijun Tian, Daniel Figeys, Hai-Ying M. Cheng, Time-of-Day- and Light-Dependent Expression of Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) in the Suprachiasmatic Nucleus Circadian Clock, PLoS One, 2014, 9, e103103
2.    Adrian Pasculescu, Erwin M. Schoof, Pau Creixellb, Yong Zheng, Marina Olhovsky, Ruijun Tian, Jonathan So, Rachel D. Vanderlaan, Tony Pawson, Rune Linding, Karen Colwill, CoreFlow: A computational platform for integration, analysis and modeling of complex biological data, J. Proteomics, 2014, 100, 167–173
3.    Jing Jin*, Karen Sison*, Chengjin Li, Ruijun Tian, Nina Jones, Dontscho Kerjachki, Masabumi Shibuya, George Fantus, Hanspeter Gerber, Napoleone Ferrara, Tony Pawson, Susan E. Quaggin, Soluble FLT1 binds lipid microdomains in podocytes to control cell morphology and glomerular barrier function, Cell, 2012, 151, 384-399 
4.    Chao Xu*, Jing Jin*, Chuanbing Bian, Robert Lam, Ruijun Tian, Ryan Weist, Linya You, Jianyun Nie, Alexey Bochkarev, Wolfram Tempel, Chris S Tan, Gregory A Wasney, Masoud Vedadi, Gerry D. Gish, Cheryl H Arrowsmith, Tony Pawson, Xiang-Jiao Yang, Jinrong Min, Sequence-Specific Recognition of a PxLPxI/L Motif by an Ankyrin Repeat Tumbler Lock, Sci. Signal., 2012, 5, ra39 
5.    James Knight, Ruijun Tian, Robin Lee, Fangjun Wang, Hanfa Zou, Lynn Megeney, Daniel Figeys, Rashmi Kothary, A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts, Skeletal Muscle, 2012, 2, 5 
6.    Houjiang Zhou, Fred Elisma, Nicholas J. Denis, Theodore G. Wright, Ruijun Tian, Hu Zhou, Weimin Hou, Hanfa Zou, Daniel Figeys, Analysis of the Subcellular Phosphoproteome Using a Novel Phosphoproteomic Reactor, J. Proteome Res., 2010, 9, 1279-1288
7.    Hu Zhou, Weimin Hou, Jean-Philippe Lambert, Ruijun Tian, Daniel Figeys, Analysis of low-abundance proteins using the proteomic reactor with pH fractionation, Talanta, 2010, 80, 1526-1531 
8.    Mohamed Abu-Farha, Fred Elisma*, Houjiang Zhou*, Ruijun Tian*, Hu Zhou*, Mehmet Selim Asmer and Daniel Figeys, Proteomics: From Technology Developments to Biological Applications, Anal. Chem., 2009, 81, 4585–4599 
9.    Fangjun Wang, Mingliang Ye, Jing Dong, Ruijun Tian, Lianghai Hu, Guanghui Han, Xinning Jiang, Ren'an Wu, Hanfa Zou, Improvement of performance in label-free quantitative proteome analysis with monolithic electrospray ionization emitter, J. Sep. Sci., 2008, 31, 2589 – 2597 
10.    Liang Zhao, Ren'an Wu, Guanghui Han, Houjiang Zhou, Lianbing Ren, Ruijun Tian, Hanfa Zou, The highly selective capture of phosphopeptides by zirconium phosphonate-modified magnetic nanoparticles for phosphoproteome analysis, J. Am. Soc. Mass. Spectrom., 2008, 19, 1176-1186
11.    Guanghui Han, Mingliang Ye, Houjiang Zhou, Xinning Jiang, Shun Feng, Xiaogang Jiang, Ruijun Tian, Dafang Wan, Hanfa Zou, Jianren Gu, Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography, Proteomics, 2008, 8, 1346-1361
12.    Houjiang Zhou, Ruijun Tian, Mingliang Ye, Songyun Xu, Shun Feng, Chensong Pan, Xiaogang  Jiang, Xin Li, Hanfa Zou, Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis, Electrophoresis, 2007, 28, 2201–2215 
13.    Xiaogang Jiang, Shun Feng, Ruijun Tian, Guanghui Han, Xinning Jiang, Mingliang Ye, Hanfa Zou, Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome analysis by using a strong cation exchange trap column, Proteomics, 2007,7,528–539
14.    Xiaogang Jiang, Xinning Jiang, Shun Feng, Ruijun Tian, Mingliang Ye, Hanfa Zou, Development of Efficient Protein Extraction Methods for Shotgun Proteome Analysis of Formalin-Fixed Tissues, J. Proteome Res., 2007, 6, 1038-1047 
15.    Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Mingliang Ye, Hanfa Zou, Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis, J. Chromatogr. A, 2007, 1171, 56-62 
16.    Mingliang Ye, Xiaogang Jiang, Shun Feng, Ruijun Tian, Hanfa Zou, Advances in chromatographic techniques and methods in shotgun proteome analysis, Trac-Trend Anal.Chem., 2007, 26, 80-84 
17.    Jiwei Hu, Chuanhui Xie, Ruijun Tian, Zhike He, Hanfa Zou, Separation of basic and acidic compounds by capillary electrochromatography using monolithic silica capillary columns with zwitterionic stationary phase, J. Sep. Sci., 2007, 30, 891-899
18.    Junming Sun, He Zhang, Ruijun Tian, Ding Ma, Xinhe Bao, Dang Sheng Su, Hanfa Zou, Ultrafast enzyme immobilization over large-pore nanoscale mesoporous silica particles, Chem. Commun., 2006, 1322-1324
19.    Jiwei Hu, Chuanhui Xie, Ruijun Tian, Zhike He, Hanfa Zou, Hybrid silica monolithic column for capillary electrochromatography with enhanced cathodic electroosmotic flow, Electrophoresis, 2006, 27, 4266–4272 
20.    Jing Dong, Chuanhui Xie, Ruijun Tian, Renan Wu, Jiwei Hu, Hanfa Zou, Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum, Electrophoresis, 2005, 26, 3452–3459 
21.    Chuanhui Xie, Jiwei Hu, Hua Xiao, Xingye Su, Jing Dong, Ruijun Tian, Zhike He, Hanfa Zou, Preparation of monolithic silica column with strong cation-exchange stationary phase for capillary electrochromatography, J. Sep. Sci., 2005, 28, 751-756 
22.    Chuanhui Xie, Jiwei Hu, Hua Xiao, Xingye Su, Jing Dong, Ruijun Tian, Zhike He, Hanfa Zou, Electrochromatographic evaluation of a silica monolith capillary column for separation of basic pharmaceuticals, Electrophoresis, 2005, 26, 790-797

Note: “*”-These authors contribute equally; “‡”-Co-corresponding authors.

Research Fields

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